Post Translational Modifications

Phosphorylation/Modifications: We are currently accepting samples for phosphorylation modification site analysis.

1. Samples for modification analysis must be coommassie stainable.

2. The more protein you can load in the gel and the more pure the protein, the better chance we have of finding modification sites.

3. Send the sequence of the protein along with the sample. We will do a theoretical trypsin digestion to determine the peptide coverage. If peptide coverage is poor with trypsin another enzyme will need to be utilized.

4. Excise gel band(s)/spot(s) with as little excess empty gel as possible.

5. Place the gel band(s)/spot(s) into a micro centrifuge tube with some dd water.

6. Fill out the Sample Submission Form .

7. Drop off or send samples and submission form to  us (Note: dry ice is not necessary).